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pEASY®-Uni Seamless Cloning and Assembly Kit

通用版同源重组无缝克隆试剂盒

目录号: CU101-01

单 价:¥1000

规格:
10 rxns
20 rxns
60 rxns
数量:
-
+
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产品详情介绍

本产品利用特殊的重组酶和同源重组的原理,可以将任意方法线性化后的载体和与其两端具有15-25 bp重叠区域的PCR片段

定向重组,可以实现最多15个片段的高效无缝拼接。

• 快速:仅需要5~15分钟反应时间。

• 简单:不受片段酶切位点的影响,无需对片段酶切。

• 高效:阳性率达95% 以上。

• 无缝:不引入额外的序列。


产品组成

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实验数据

组装效率高

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14 kb 长载体组装

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低浓度多片段成功连接(片段投入量为常规投入量的 1/10)

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References
  • Xu Y, Zhu T F. Mirror-image T7 transcription of chirally inverted ribosomal and functional RNAs[J]. Science, 2022.(IF 63.71)

  • Deng Z, Pu L, Deng K, et al. Targeting TFAP2β condensation suppresses the development of esophageal squamous cell carcinoma[J]. Cell, 2025.(IF 42.50)

  • Zhu Z, Wang Y, Liu S, et al. Genomic atlas of 8,105 accessions reveals stepwise domestication, global dissemination, and improvement trajectories in soybean[J]. Cell, 2025.(IF 42.50)

  • Shi J Y, Mei C,Ge F Y, et,al. Resistance to Striga parasitism through reduction of strigolactone exudation[J].Cell, 2025.(IF 42.50)

  • Bai X, Sun P, Wang X, et al. Structure and dynamics of the EGFR/HER2 heterodimer[J]. Cell Discovery, 2023.(IF 33.50)

  • Wang H, Yang J, Cai Y, et al. Macrophages suppress cardiac reprogramming of fibroblasts in vivo via IFN-mediated intercellular self-stimulating circuit[J]. Protein & Cell, 2024.(IF 21.10)

  • Xu J, Liang Y, Li N, et al. Clathrin-associated carriers enable recycling through a kiss-and-run mechanism[J]. Nature Cell Biology, 2024.(IF 17.30)

  • Liu S, Fan L, Liu Z, et al. A Pd1–Ps–P1 feedback loop controls pubescence density in soybean[J]. Molecular plant, 2020.(IF 17.10)

  • Shi C, Yang X, Hou Y, et al. USP15 promotes cGAS activation through deubiquitylation and liquid condensation[J]. Nucleic Acids Research, 2022.(IF 16.60)

  • Li B, Zhu L, Lu C, et al. circNDUFB2 inhibits non-small cell lung cancer progression via destabilizing IGF2BPs and activating anti-tumor immunity[J]. Nature communications, 2021.(IF 14.70)

  • Wang J, An Z, Wu Z, et al. Spatial organization of PI3K-PI (3, 4, 5) P3-AKT signaling by focal adhesions[J]. Molecular Cell, 2024.(IF 14.50)

  • Wang H, Yang J, Cai Y, et al. Macrophages suppress cardiac reprogramming of fibroblasts in vivo via IFN-mediated intercellular self-stimulating circuit[J]. Protein & Cell, 2024.(IF 13.60)

  • Bai X, Sun P, Wang X, et al. Structure and dynamics of the EGFR/HER2 heterodimer[J]. Cell Discovery, 2023.(IF 13.00)

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