pEASY®-Uni Seamless Cloning and Assembly Kit
通用版同源重组无缝克隆试剂盒
| 目录号 | 规格 | 单价 |
|---|---|---|
| CU101-01 | 10 rxns | 1000 |
| CU101-02 | 20 rxns | 1800 |
| CU101-03 | 60 rxns | 4330 |
产品详情介绍
本产品利用特殊的重组酶和同源重组的原理,可以将任意方法线性化后的载体和与其两端具有15-25 bp重叠区域的PCR片段
定向重组,可以实现最多15个片段的高效无缝拼接。
• 快速:仅需要5~15分钟反应时间。
• 简单:不受片段酶切位点的影响,无需对片段酶切。
• 高效:阳性率达95% 以上。
• 无缝:不引入额外的序列。



组装效率高

14 kb 长载体组装

低浓度多片段成功连接(片段投入量为常规投入量的 1/10)

Xu Y, Zhu T F. Mirror-image T7 transcription of chirally inverted ribosomal and functional RNAs[J]. Science, 2022.(IF 63.71)
Deng Z, Pu L, Deng K, et al. Targeting TFAP2β condensation suppresses the development of esophageal squamous cell carcinoma[J]. Cell, 2025.(IF 42.50)
Zhu Z, Wang Y, Liu S, et al. Genomic atlas of 8,105 accessions reveals stepwise domestication, global dissemination, and improvement trajectories in soybean[J]. Cell, 2025.(IF 42.50)
Shi J Y, Mei C,Ge F Y, et,al. Resistance to Striga parasitism through reduction of strigolactone exudation[J].Cell, 2025.(IF 42.50)
Bai X, Sun P, Wang X, et al. Structure and dynamics of the EGFR/HER2 heterodimer[J]. Cell Discovery, 2023.(IF 33.50)
Wang H, Yang J, Cai Y, et al. Macrophages suppress cardiac reprogramming of fibroblasts in vivo via IFN-mediated intercellular self-stimulating circuit[J]. Protein & Cell, 2024.(IF 21.10)
Xu J, Liang Y, Li N, et al. Clathrin-associated carriers enable recycling through a kiss-and-run mechanism[J]. Nature Cell Biology, 2024.(IF 17.30)
Liu S, Fan L, Liu Z, et al. A Pd1–Ps–P1 feedback loop controls pubescence density in soybean[J]. Molecular plant, 2020.(IF 17.10)
Shi C, Yang X, Hou Y, et al. USP15 promotes cGAS activation through deubiquitylation and liquid condensation[J]. Nucleic Acids Research, 2022.(IF 16.60)
Li B, Zhu L, Lu C, et al. circNDUFB2 inhibits non-small cell lung cancer progression via destabilizing IGF2BPs and activating anti-tumor immunity[J]. Nature communications, 2021.(IF 14.70)
Wang J, An Z, Wu Z, et al. Spatial organization of PI3K-PI (3, 4, 5) P3-AKT signaling by focal adhesions[J]. Molecular Cell, 2024.(IF 14.50)
Wang H, Yang J, Cai Y, et al. Macrophages suppress cardiac reprogramming of fibroblasts in vivo via IFN-mediated intercellular self-stimulating circuit[J]. Protein & Cell, 2024.(IF 13.60)
Bai X, Sun P, Wang X, et al. Structure and dynamics of the EGFR/HER2 heterodimer[J]. Cell Discovery, 2023.(IF 13.00)




