TransLvTM Lentivirus qPCR Titration Kit
慢病毒qPCR滴度检测试剂盒
目录号： FV201-01
单 价：￥2860
产品详情介绍
TransLvTM Lentivirus qPCR Titration Kit提供了一种利用qPCR方法检测慢病毒转导细胞中整合的前病毒序列拷贝数的方法。该方法简单快速、灵敏高效，兼容第二代和第三代HIV-1慢病毒包装载体。慢病毒包装后转导易感细胞 (如HT1080，HEK-293T等)，提取慢病毒转导细胞基因组，进行qPCR反应，通过标准曲线Ct值计算整合的前病毒拷贝数，进而得到确定的慢病毒滴度。
以梯度稀释的标准品为模板进行扩增得到标准曲线，结果显示，TransGen产品扩增效率高，线性关系好。
慢病毒滴度检测介绍视频
Shen H, Yang M, Li S, et al. Mouse totipotent stem cells captured and maintained through spliceosomal repression[J]. Cell, 2021.(IF 38.50)
Du P, Li N, Xiong X, et al. A bivalent vaccine containing D614G and BA. 1 spike trimer proteins or a BA. 1 spike trimer protein booster shows broad neutralizing immunity[J]. Journal of Medical Virology, 2022.(IF 20.69)
Zhou Z, Du P, Yu M, et al. Assessment of infectivity and the impact on neutralizing activity of immune sera of the COVID-19 variant, CAL. 20C[J]. Signal Transduction and Targeted Therapy, 2021.(IF 18.18)
Bi J, Wang H, Pei H, et al. A novel and secure pseudovirus reporter system based assay for neutralizing and enhancing antibody assay against Marburg virus[J]. Frontiers in microbiology, 2022.(IF 6.06)
Zheng B, Zhang W, Zhou D, et al. Lentiviral Dendritic Cell Vaccine Targeting Claudin-18.2 Elicits Potent Antitumor Immunity Against Gastric Cancer[J]. Cancers, 2026.(IF 4.80)
Du P, Li M, Wei G, et al. JN. 1: enhanced immune evasion ability propels it to become the predominant strain in China, unlikely to trigger pandemic similar to late 2022[J]. Frontiers in public health, 2024.(IF 3.00)
