TransScript® Uni All-in-One First-Strand cDNA Synthesis SuperMix for qPCR (One-Step gDNA Removal)
耐高温全预混第一链cDNA合成试剂盒（一步法去除gDNA）（用于qPCR）
目录号： AU341-02
单 价：￥6110
产品详情介绍
TransScript® Uni All-in-One First-Strand cDNA Synthesis SuperMix for qPCR (One-Step gDNA Removal)包含反转录反应所需的全部成分(TransScript® Uni RT, RNase Inhibitor, Anchored Oligo(dT)20 Primer, Random Primer(N9), dNTPs, Buffer)，可在同一管反应中实现cDNA 合成和去除基因组DNA。反应时，只需加入5×All-in-One Reaction Mix for qPCR、TransScript® Uni All-in-One Enzyme Mix、模板RNA和水，在42℃-65℃条件下即可高效地合成第一链cDNA，同时去除RNA模板中残留的基因组DNA 。另配有20×No RT Control Mix，用于配制无反转录酶的对照，判断qPCR模板是否来自cDNA。该产品操作简便，降低操作过程中的污染机率。cDNA产物只适用于qPCR，不适用于常规PCR。
线性范围广
使用 TransGen 产品，以不同起始量 RNA（500 ng-50 pg，10 倍梯度稀释）为模板进行反转录后定量扩增 GAPDH 基因。结果表明， TransGen 产品具有强的反转录效率，模板起始量可达 50 pg。
适用物种范围广
使用 Company V1、Company V2、TransGen 产品，以不同物种 RNA 为模板进行反转录后定量扩增不同基因。结果表明， TransGen 产品可适用于多种物种。
基因组DNA去除能力强
使用 TransGen 产品，以 200 ng RNA、 200 ng RNA+200 ng gDNA、 200 ng gDNA 为模板进行反转录后定量扩增不同基因。结果表明， TransGen 产品具有强的 gDNA 去除能力。
批间稳定性好
使用不同批次 TransGen 产品，以不同物种 RNA 为模板进行反转录后定量扩增不同基因。结果表明，不同批次间 TransGen 产品的稳定性好。
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