Easy II Protein Quantitative Kit (BCA)
蛋白定量试剂盒 (BCA)
目录号： DQ111-01
单 价：￥440
产品详情介绍
BCA蛋白定量方法是目前最常用的两种蛋白定量方法之一，其原理是在碱性条件下，蛋白质中的肽键能将Cu2+还原成Cu1+(双缩脲反应)，且Cu2+的还原量与总蛋白量成正比，生成的Cu1+能够与BCA形成稳定的紫色化合物。与Bradford方法相比，BCA蛋白定量方法更加客观，普通的肽键即可发生颜色反应，亦可兼容高浓度的SDS(5%)，Triton X-100(5%)，NP 40(5%)，CHAPS(5%)，Tween 20，60，80(5%)，其弊端是易受某些还原剂(如二硫苏糖醇和β-硫基乙醇等)、螯合剂(EDTA，EGTA)和高浓度缓冲液的影响，但这些干扰作用可以通过对蛋白质样品的稀释进行消除。
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