产品详情介绍
GelStain是一种灵敏、稳定和相对安全的荧光核酸染色试剂。它可以替代高毒性染色剂－溴化乙锭(EB)，用于琼脂糖凝胶或聚丙烯酰胺凝胶中dsDNA、ssDNA和RNA的染色。GelStain的灵敏度远高于EB，并且不需要脱色。GelStain和EB有相同的光谱特性。
特点：
• 无毒性：GelStain独特的油性和大分子特点使其不能穿透细胞膜进入细胞内，艾姆斯氏实验结果也表明，该染料的诱变性远远小于EB。
• 灵敏度高：适用于不同大小的片段电泳染色，对核酸迁移率的影响小于SYBR Green I。
• 稳定性高：适用于使用微波或其它加热方法制备琼脂糖凝胶；室温下在酸性、碱性环境下极其稳定，耐光性强。
• 信噪比高：样品荧光信号强，背景信号低。
• 操作简单：与EB一样，在预制胶和电泳过程中染料不降解；而电泳后染色过程也只需30分钟且无需脱色或冲洗，即可直接用紫外凝胶透射仪观察。
• 适用范围广：可选择电泳前染色（胶染法）或电泳后染色（泡染法）；适用于琼脂糖凝胶或聚丙烯酰胺凝胶电泳；可用于dsDNA、ssDNA或RNA染色。
• 无需改变滤光片及观察装置：标准的EB滤光片或SYBR滤光片都适用，使用与观察EB染色相同的普通紫外凝胶透射仪观察即可，在300 nm紫外光附近可得到最佳激发。
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