产品详情介绍
该载体通过自杀基因表达与否筛选阳性重组子。当载体与片段连接成功时，自杀基因无法正确表达，包含重组子的细胞可以正常生长；当载体与片段连接不成功时，自杀基因正确表达，包含载体的细胞无法生长，即“Zero”背景。适用于TA克隆。
Chen W, Chen L, Zhang X, et al. Convergent selection of a WD40 protein that enhances grain yield in maize and rice[J]. Science, 2022.(IF 63.71)
He Y, Zhu M, Wang L, et al. Programmed self-elimination of the CRISPR/Cas9 construct greatly accelerates the isolation of edited and transgene-free rice plants[J]. Molecular plant, 2018.(IF 9.33)
Zhao Z, Zhang Z, Li J, et al. Sustained TNF-α stimulation leads to transcriptional memory that greatly enhances signal sensitivity and robustness[J]. Elife, 2020.(IF 7.08)
Zhang L, Ma F, Duan G, et al. MIKC*-type MADS transcription factors control JINGUBANG expression and the degree of pollen dormancy in Arabidopsis[J]. Plant Physiology, 2025.(IF 6.90)
Cao Y, Yan D, Zhou H, et al. Achieving precise dual detection: One-tube reverse transcription-recombinase aided amplification (RT-RAA) combined with lateral flow strip (LFS) assay for RNA and DNA target genes from pepper mild mottle virus and Colletotrichum species in crude plant samples[J]. Talanta, 2025.(IF 6.10)
Chang J, Yang X, Zhang T, et al. High-Throughput Screening to Identify Novel Compounds Affecting the Genome Editing Efficiency of CRISPR System[J]. Molecules, 2025.(IF 4.60)
