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Fast Mutagenesis System

Fast定点突变试剂盒

目录号: FM111-01

单 价:¥790

规格:
10 rxns
20 rxns
数量:
-
+
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产品详情介绍

本产品以甲基化的质粒为模板,采用部分重叠引物 (均含突变点) 设计,使用2×TransStart® FastPfu Fly PCR SuperMix扩增,扩增产物用DMT限制性内切酶消化甲基化质粒模板后,转化具有降解甲基化质粒模板的感受态细胞。本产品具有突变效率高,操作简便的优点。



产品组成

References
  • Liu X, Liu Z, Wu Z, et al. Resurrection of endogenous retroviruses during aging reinforces senescence[J]. Cell, 2023.(IF 66.85)

  • Yu Y, Tang W, Lin W, et al. ABLs and TMKs are co-receptors for extracellular auxin[J]. Cell, 2023.(IF 64.50)

  • Wang K, Zhang Z, Hang J, et al. Microbial-host-isozyme analyses reveal microbial DPP4 as a potential antidiabetic target[J]. Science, 2023.(IF 56.90)

  • Zeng Y, Zhang H W, Wu X X, et al. Structural basis of exoribonuclease-mediated mRNA transcription termination[J]. Nature, 2024.(IF 50.50)

  • Chen C C, Yu Z P, Liu Z, et al. Chanoclavine synthase operates by an NADPH-independent superoxide mechanism[J]. Nature, 2025.(IF 48.50)

  • Chen Y, Chen Z X, Wang H, et al. A cGAS-mediated echanism in naked mole-rats potentiates DNA repair and delays aging[J]. Science, 2025.(IF 45.80)

  • Tian Y, Chen Z H, Wu P, et al. MIR497HG-Derived miR-195 and miR-497 Mediate Tamoxifen Resistance via PI3K/AKT Signaling in Breast Cancer[J].Advanced Science, 2023.(IF 17.52)

  • Zhang L, Gao T, Li Z, et al. Alkylated DNA repair by a novel HhH-GPD family protein from Crenarchaea[J]. Nucleic Acids Research, 2025.(IF 16.70)

  • Ruan T, Ma J, Shen G, et al. A novel dual histone mark reader ZCWPW2 regulates meiotic recombination through lactylation and transcriptional regulation in humans and mice[J]. Nucleic Acids Research, 2026.(IF 13.10)

  • Lu Y, Jia S, Guo R, et al. SHH1 cooperates with the DNA methylation reader MBD7 to suppress transcriptional silencing of promoter-methylated genes in Arabidopsis[J]. Plant Communications, 2026.(IF 11.60)

  • Yuan T, Ye Z, Mei S, et al. PRMT1-mediated methylation of UBE2m promoting calcium oxalate crystal-induced kidney injury by inhibiting fatty acid metabolism[J]. Cell Death & Disease, 2025.(IF 9.60)

  • Wu S, Wang B, Li H, et al. Targeting STING elicits GSDMD-dependent pyroptosis and boosts anti-tumor immunity in renal cell carcinoma[J]. Oncogene, 2026.(IF 7.30)

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