TransScript® One-Step gDNA Removal and cDNA Synthesis SuperMix
TransScript一步法去除gDNA及第一链cDNA合成试剂盒
目录号： AT311-02
单 价：￥1160
产品详情介绍
本产品以RNA为模板，在同一反应体系中，合成第一链cDNA的同时去除RNA模板中残留的基因组DNA。反应结束后，只需在85℃加热2分钟，即可同时失活TranScript® RT/RI与gDNA Remover。
使用前请将各组分点甩离心
产品稳定性
使用不同批次产品分别以人总RNA为模板，进行RT-PCR检测，1.0%琼脂糖凝胶电泳分析反转录效果。
使用不同批次产品分别以人100 ng总RNA、人100 ng总RNA+200 ng gDNA、200 ng gDNA为模板，进行RT-PCR检测，1.0%琼脂糖凝胶电泳分析模板DNA去除效果；qRT-PCR检测18S RNA表达量。
与竞品的比较
使用TransGen、Company TA和Company TH产品，分别以人100 ng总RNA、人100 ng总RNA+200 ng gDNA、200 ng gDNA为模板，进行RT-PCR检测， 1.0%琼脂糖凝胶电泳分析模板DNA去除效果；qRT-PCR检测18S RNA表达量。
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