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快速内切酶和修饰酶

快速内切酶和修饰酶

DNA 分子量标准

DNA 分子量标准

蛋白提取、纯化及检测

蛋白提取、纯化及检测

ELISA

ELISA

TransZol  Up Plus RNA Kit

目录号: ER501-01-V2

单 价:¥1300

规格:
100 rxns
数量:
-
+
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产品详情介绍

本试剂盒适用于从细胞和组织中提取总RNA,用TransZol Up裂解样品,加入RNA Extraction Agent后,溶液分为无色水相和粉红色有机相,RNA在水相中;用硅胶膜离心柱特异吸附水相中的RNA,与其它总RNA提取方法相比,既具有TransZol Up裂解能力强、提取量高,应用范围广的优点,又具有离心柱提取RNA纯度高的优点。


产品组成

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实验数据

RNA提取效率高

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使用TransGen ER501产品,分别以REA和氯仿作为抽提试剂,提取等量的Hela细胞的RNA,琼脂糖凝胶电泳分析提取效果。结果表明, TransGen 产品RNA提取效率高,REA与氯仿提取效果相当。


对下游实验无扩增抑制

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使用TransGen ER501产品,分别以REA和氯仿作为抽提试剂,提取Hela细胞的RNA,用TransGen一步法RT-PCR产品 (AT411)分析扩增效果。结果表明,使用REA作为抽提试剂提取的RNA对下游PCR实验无扩增抑制。

image.png

使用TransGen ER501产品,分别以氯仿和REA作为抽提试剂,提取Hela细胞、烟草、小鼠组织的RNA,分别用TransGen一步法RT-qPCR产品(AQ211)和两步法RT-qPCR产品 (AUQ)分析扩增效果。结果表明,使用REA作为抽提试剂提取的RNA对下游qPCR实验无扩增抑制。


DNA残留量低

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使用TransGen ER501产品,分别以氯仿和REA作为抽提试剂 ,以提取的Hela细胞RNA为模板,人gDNA为引物,用染料法qPCR产品(AQ601)分析DNA残留量。结果表明,使用REA作为抽提试剂提取的RNA中DNA残留量低。


适用多物种RNA的提取

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使用TransGen ER501产品,分别以REA和氯仿作为抽提试剂,提取293T细胞、小鼠肝组织、烟草的RNA,琼脂糖凝胶电泳分析提取效果。结果表明,TransGen产品可用于提取多物种RNA。



References

1 Ye X, Xu L, Li X, et al. miR-34 modulates wing polyphenism in planthopper[J]. PLoS Genetics, 2019.(IF 5.22)

2 Zhao Z, Bao X, Zhang Z, et al. Novel phloroglucinol derivative Compound 21 protects experimental autoimmune encephalomyelitis rats via inhibiting Th1/Th17 cell infiltration[J]. Brain, Behavior, and Immunity, 2020.(IF 6.17)

3 Zeng Y H, Cai Z H, Zhu J M, et al. Two hierarchical LuxR-LuxI type quorum sensing systems in Novosphingobium activate microcystin degradation through transcriptional regulation of the mlr pathway[J]. Water Research, 2020.(IF 9.13)

4 Zhang Y, Liu J, Liu J L. The atlas of cytoophidia in Drosophila larvae[J]. Journal of genetics and genomics, 2020.(IF 5.06)

5 Tan Y, Yan X, Sun J, et al. Genome‐wide enhancer identification by massively parallel reporter assay in Arabidopsis[J]. The Plant Journal, 2023.(IF 7.20)

6 Wang B, Tang X, Yao L, et al. Disruption of USP9X in macrophages promotes foam cell formation and atherosclerosis[J]. The Journal of Clinical Investigation, 2022.(IF 14.80)

7 Li X, Zhou L, Gao B Q, et al. Highly efficient prime editing by introducing same-sense mutations in pegRNA or stabilizing its structure[J]. Nature Communications, 2022.(IF 17.69)

8 Du P, Li N, Xiong X, et al. A bivalent vaccine containing D614G and BA. 1 spike trimer proteins or a BA. 1 spike trimer protein booster shows broad neutralizing immunity[J]. Journal of Medical Virology, 2022.(IF 20.69)

9 Guo Z, Cao H, Zhao J, et al. A natural uORF variant confers phosphorus acquisition diversity in soybean[J]. Nature Communications, 2022.(IF 17.69)

10 Zhao Z, Ning J, Bao X, et al. Fecal microbiota transplantation protects rotenone-induced Parkinson’s disease mice via suppressing inflammation mediated by the lipopolysaccharide-TLR4 signaling pathway through the microbiota-gut-brain axis[J]. Microbiome, 2021.(IF 16.83)

11 Zhang M, Li F D, Li K, et al. Functional characterization and structural basis of an efficient di-C-glycosyltransferase from Glycyrrhiza glabra[J]. Journal of the American Chemical Society, 2020.(IF 14.61)

12 Wang L, Xue W, Zhang H, et al. Eliminating base-editor-induced genome-wide and transcriptome-wide off-target mutations[J]. Nature Cell Biology, 2021.(IF 20.04)

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