T7 High Efficiency Transcription Kit
T7高效体外转录试剂盒
| 目录号 | 规格 | 单价 |
|---|---|---|
| JT101-01 | 25 rxns | 1160 |
| JT101-02 | 100 rxns | 3700 |
产品详情介绍
本试剂盒进行了体外转录体系的优化，利用T7 RNA Polymerase，以含有T7启动子的超螺旋质粒DNA或线性DNA为模板，对T7启动子下游的DNA序列进行高效转录。本产品适用于制备长度超过6000 nt的高浓度RNA，使用1 μg DNA模板可在20 μl体系生成150~280 μg的RNA (如果想获得毫克级的RNA产物，可平行放大反应体系)。制备的RNA可用于体外翻译、RNase保护实验、RNA剪切以及杂交探针标记等。
转录特异性好
转录产量高
兼容不同类型DNA模板
兼容化学修饰核苷酸
同类产品对比
荧光镜检和流式细胞分选结果表明，利用TransGen mRNA体外合成系列产品(JT101)合成的mRNA毒性低、稳定性好、翻译效率高，在不同类型的哺乳动物细胞中成功且稳定表达。
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7.Hu R, Li M, Chen S, et al. Sniffer restricts arboviral brain infections by regulating ROS levels and protecting blood-brain barrier integrity in Drosophila and mosquitoes[J]. PLoS pathogens, 2024.(IF 5.50)
