TransTaq® DNA Polymerase High Fidelity (HiFi) (含 2.5 mM dNTPs)
长片段/复杂模板扩增 DNA 聚合酶(含 2.5 mM dNTPs)
| 目录号 | 规格 | 单价 |
|---|---|---|
| AP131-11 | 250 units | 330 |
| AP131-12 | 500 units | 620 |
| AP131-13 | 6×500 units | 3330 |
产品详情介绍
TransTaq® DNA Polymerase High Fidelity (TransTaq® HiFi DNA Polymerase)是以TransTaq®-T DNA Polymerase为主的复合酶。TransTaq® HiFi DNA Polymerase具有5'→3' DNA聚合酶活性，5'→3'外切酶活性和3'→5'外切酶活性。不同模板使用不同Buffer实现高效扩增。TransTaq® HiFi Buffer I 适用于基因组DNA的扩增。TransTaq® HiFi Buffer II 适用于 λDNA，cDNA和Plasmid DNA的扩增。
• 保真性是EasyTaq® DNA Polymerase 的18倍。
• 延伸速度为1-2 kb/min。
• 扩增产物3' 端带"A"碱基，可直接克隆于pEASY®-T载体中。
• 基因组DNA片段的扩增 (≤15 kb)。
Liu Y, Cao Z, Wang Y, et al. Genome-wide screening for functional long noncoding RNAs in human cells by Cas9 targeting of splice sites[J].Nature Biotechnology, 2018.(IF 54.91)
Li S, Li X, Xue W, et al. Screening for functional circular RNAs using the CRISPR–Cas13 system[J]. Nature methods, 2021.(IF 30.82)
Ran R, Li L, Cheng P, et al. High frequency of melanoma in cdkn2b-/-/tp53-/-Xenopus tropicalis[J]. Theranostics, 2024.(IF 13.30)
Hao T, Xie Z, Wang M, et al. An anaerobic bacterium host system for heterologous expression of natural product biosynthetic gene clusters[J]. Nature communications, 2019.(IF 10.70)
Di Y Q, Han X L, Kang X L, et al. Autophagy triggers CTSD (cathepsin D) maturation and localization inside cells to promote apoptosis[J]. Autophagy, 2021.(IF 9.77)
Du J, Wang X, Li Y, et al. DHA exhibits synergistic therapeutic efficacy with cisplatin to induce ferroptosis in pancreatic ductal adenocarcinoma via modulation of iron metabolism[J]. Cell Death & Disease, 2021.(IF 8.40)
Cao J, Huang C, Liu J, et al. Comparative Genomics and Functional Studies of Putative m6A Methyltransferase (METTL) Genes in Cotton[J]. International Journal of Molecular Sciences, 2022.(IF 6.21)
Zhou L, Zou W, Zheng S, et al. Toxoplasma gondii hijacks host S100A6 to enhance cell migration and brain cyst formation[J]. Communications Biology, 2026.(IF 5.10)
