TransScript® II All-in-One First-Strand cDNA Synthesis SuperMix for qPCR (One-Step gDNA Removal)
TransScript II All-in-One 第一链cDNA合成试剂盒（一步法去除gDNA）（用于qPCR）
| 目录号 | 规格 | 单价 |
|---|---|---|
| AH341-01 | 50 rxns×20 µl体系 | 2420 |
产品详情介绍
本产品含有反转录反应所需的全部试剂(TransScript® II RT, RNase Inhibitor, Anchored Oligo(dT)20 Primer, Random Primer(N9), dNTPs, Buffer)，浓度为5×。反应时，只需加入gDNA Remover、模板RNA和水，在42℃-55℃条件下即可高效地合成第一链cDNA，同时去除RNA模板中残留的基因组DNA。另配有5×TransScript® II All-in-One No-RT Control SuperMix for qPCR，用于配制无反转录酶的对照，判断qPCR模板是否来自cDNA。该产品操作简便，降低操作过程中的污染机率。cDNA只适用于qPCR，不适用于常规PCR。
使用前请将各组分点甩离心
Wang S, Liu C, Zhang C, et al. A-to-I RNA-Editing: an epigenetic hallmark cannot be ignored in silencing the tumor microenvironment and is promising in predicting immunotherapy response for esophageal squamous cell carcinoma[J]. Gastroenterology, 2023.(IF 29.40)
Liu C, Zheng S, Wang Z, et al. KRAS‐G12D mutation drives immune suppression and the primary resistance of anti‐PD‐1/PD‐L1 immunotherapy in non‐small cell lung cancer[J]. Cancer Communications, 2022.(IF 15.28)
Wang S, Zheng S, Liu C, et al. Inhibiting ADAR1‐Mediated Excessive Epigenetic A‐to‐I RNA Editing Improves the Immune Microenvironment and Increases Sensitivity to Immunotherapy in Lung Adenocarcinoma[J]. MedComm, 2026.(IF 14.10)
Li H S, Liu C M, Zheng S F, et al. RANKL/PD-1 dual blockade demonstrates survival benefit for patients with advanced lung adenocarcinoma harboring KRAS mutations[J]. Cell Reports Medicine, 2025.(IF 10.60)
Zhang F, Chen S, Cui T, et al. Novel function of the PsDMAP1 protein in regulating the growth and pathogenicity of Phytophthora sojae[J]. International Journal of Biological Macromolecules, 2023.(IF 8.20)
Li H, Lin Y, Xin Y, et al. Fatty acid binding protein 4 (FABP4): a key player in neuroinflammation and neuropathic pain[J]. Free Radical Biology and Medicine, 2026.(IF 8.00)
Tian L, Liu C, Zheng S, et al. KEAP1 mutations as key crucial prognostic biomarkers for resistance to KRAS-G12C inhibitors[J]. Journal of Translational Medicine, 2025.(IF 7.50)
Huang Z, Liu X, Zhou X, et al. Asparaginase and autophagy inhibitors effectively remove senescent cells by synergistically limiting asparagine supply[J]. Aging Cell, 2025.(IF 7.10)
Jiu X, Li W, Liu Y, et al. TREM2, a critical activator of pyroptosis, mediates the anti-tumor effects of piceatannol in uveal melanoma cells via caspase 3/GSDME pathway[J]. International Journal of Molecular Medicine, 2024.(IF 5.70)
Tang W, Zhang L, Cao P, et al. Rbm25 governs embryonic stem cell identity and fate through transcriptional regulation of pluripotency and epigenetic programs[J]. Stem Cell Reports, 2026.(IF 5.60)
