ProteinIso® Ni-NTA Resin
Ni-NTA Resin 蛋白纯化
| 目录号 | 规格 | 单价 |
|---|---|---|
| DP101-01 | 5 ml | 570 |
| DP101-02 | 25 ml | 2420 |
产品详情介绍
ProteinIso® Ni-NTA Resin是螯合金属Ni2+而形成的一种亲和层析介质。NTA能够通过四个位点牢固地螯合Ni2+，从而减少纯化过程中Ni2+泄漏到蛋白样品中。本产品对His标签蛋白具有特异吸附能力，从而使His标签蛋白结合在Ni-NTA纯化介质上，未结合的蛋白被洗涤下去。结合在介质上的蛋白经过一定浓度的咪唑或者pH低缓冲液温和洗脱下来，从而得到高纯度的目的蛋白。本产品具有吸附容量大、选择性好、通透性强、易于再生等优点，适用于在非变性或变性条件下纯化任何表达系统表达的His标签重组蛋白。
1.Zhou L, Liu H, Liu S, et al. Structures of sperm flagellar doublet microtubules expand the genetic spectrum of male infertility[J]. Cell, 2023.(IF 65.00)
2.Guo L, Zhao Y, Zhang Q, et al. Stochastically multimerized ParB orchestrates DNA assembly as unveiled by single-molecule analysis[J]. Nucleic Acids Research, 2022.(IF 19.16)
3.Zhou C J, Wang X Y, Dong Y H, et al. CENP-F-dependent DRP1 function regulates APC/C activity during oocyte meiosis I[J]. Nature communications, 2022,(IF 17.69)
4.Zhang Q, Chen Z, Wang F, et al. Efficient DNA interrogation of SpCas9 governed by its electrostatic interaction with DNA beyond the PAM and protospacer[J]. Nucleic Acids Research, 2021.(IF 16.97)
5.Zhou B, Luo Q, Shen Y, et al. Coordinated regulation of vegetative phase change by brassinosteroids and the age pathway in Arabidopsis[J]. Nature Communications, 2023.(IF 16.60)
6.Wu W K, Nie G B, Lin J L, et al. Regulation of glandular size and phytoalexin biosynthesis by a negative feedback loop in cotton[J]. Advanced Science, 2024,(IF 14.30)
7.Zhang Y, Gao Y, Wang H L, et al. Verticillium dahliae secretory effector PevD1 induces leaf senescence by promoting ORE1-mediated ethylene biosynthesis[J]. Molecular plant, 2021.(IF 13.16)
8.Wei Y, Liu Z, Lv T, et al. Ethylene enhances MdMAPK3-mediated phosphorylation of MdNAC72 to promote apple fruit softening[J]. The Plant Cell, 2023.(IF 11.60)
9.Zheng W, Li S, Shi Z, et al. Recombinant ferritin-based nanoparticles as neoantigen carriers significantly inhibit tumor growth and metastasis[J]. Journal of Nanobiotechnology, 2024.(IF 10.60)
10.Liu Y, Yu T F, Li Y T, et al. Mitogen‐activated protein kinase TaMPK3 suppresses ABA response by destabilising TaPYL4 receptor in wheat[J]. New Phytologist, 2022.(IF 10.32)
11.Hu C, Wu S, Li J, et al. Herbivore‐induced Ca2+ signals trigger a jasmonate burst by activating ERF16‐mediated expression in tomato[J]. New Phytologist, 2022.(IF 10.32)
12.Gao Y, Li Z, Yang C, et al. Pseudomonas syringae activates ZAT18 to inhibit salicylic acid accumulation by repressing EDS1 transcription for bacterial infection[J]. New Phytologist, 2022.(IF 10.15)
13.Gao J, Zhao B R, Zhang H, et al. Interferon functional analog activates antiviral Jak/Stat signaling through integrin in an arthropod[J]. Cell reports, 2021,(IF 9.42)
14.Li P, Tan X, Liu R, et al. QTL detection and candidate gene analysis of grape white rot resistance by interspecific grape (Vitis vinifera L.× Vitis davidii Foex.) crossing[J]. Horticulture Research, 2023.(IF 8.70)
15.Chen K, Sun W, Zhong M, et al. Single-molecule assay guided crRNA optimization enhances specific microRNA detection by CRISPR-Cas12a[J]. Sensors and Actuators B: Chemical, 2024.(IF 8.40)
16.Fu W, Liu Y, Liu F, et al. A novel autoantibody induced by bacterial biofilm conserved components aggravates lupus nephritis[J]. Frontiers in Immunology, 2021.(IF 7.56)
17.Xu Y, Liu Z, Lv T, et al. Exogenous Ca2+ promotes transcription factor phosphorylation to suppress ethylene biosynthesis in apple[J]. Plant Physiology, 2023.(IF 7.40)
18.Song M, Fan X, Chen J, et al. OsNAR2. 1 interaction with OsNIT1 and OsNIT2 functions in root-growth responses to nitrate and ammonium[J]. Plant physiology, 2020,(IF 6.90)
19.Wang F P, Zhao P P, Zhang L, et al. The VvWRKY37 Regulates Bud Break in Grape Vine Through ABA-Mediated Signaling Pathways[J]. Frontiers in Plant Science, 2022.(IF 6.62)
20.Wang Z, Wang S, Xu Z, et al. Highly promiscuous flavonoid 3-O-glycosyltransferase from Scutellaria baicalensis[J]. Organic letters, 2019.(IF 6.55)
21.Xiao M, Wang B, Feng Y, et al. Three candidate 2-(2-phenylethyl) chromone-producing type III polyketide synthases from Aquilaria sinensis (Lour.) Gilg have multifunctions synthesizing benzalacetones, quinolones and pyrones[J]. Industrial Crops and Products, 2022.(IF 6.45)
22.Li X J, Yang L, Li D, et al. Pathogen-specific binding soluble down syndrome cell adhesion molecule (Dscam) regulates phagocytosis via membrane-bound Dscam in crab[J]. Frontiers in immunology, 2018,(IF 6.43)
23.Liu C, Shen N, Zhang Q, et al. Magnaporthe oryzae transcription factor MoBZIP3 regulates appressorium turgor pressure formation during pathogenesis[J]. International Journal of Molecular Sciences, 2022.(IF 6.21)
24.Liu S, Zhang M, Bao Y, et al. Characterization of a Highly Selective 2 ″-O-Galactosyltransferase from Trollius chinensis and structure-guided engineering for improving UDP-glucose selectivity[J]. Organic Letters, 2021.(IF 6.00)
25.Chen K, Hu Z, Song W, et al. Diversity of O-glycosyltransferases contributes to the biosynthesis of flavonoid and triterpenoid glycosides in Glycyrrhiza uralensis[J]. ACS Synthetic Biology, 2019.(IF 5.57)
26.Liu X, Zhu X, Wei X, et al. The wheat LLM-domain-containing transcription factor TaGATA1 positively modulates host immune response to Rhizoctonia cerealis[J]. Journal of Experimental Botany, 2020.(IF 5.36)
