文章信息
文章题目：Time-resolved reprogramming of single somatic cells into totipotent states during plant regeneration
期刊：Cell
发表时间：2025 年 9 月 16 日
主要内容：山东农业大学张宪省教授和苏英华教授研究团队首次完整揭示了单个植物体细胞如何通过基因重编程“改变命运”，最终发育为完整植株的全过程。该成果不仅破解了困扰科学界百余年的“植物细胞全能性”机制之谜，也为作物遗传改良与高效再生提供了全新理论支撑。
原文链接：
https://doi.org/10.1016/j.cell.2025.08.031
使用TransGen产品：
EasyScript® One-Step gDNA Removal and cDNA Synthesis SuperMix (AE311)
TransStart® Green qPCR SuperMix (AQ101)
ProteinFind® Anti-HA Mouse Monoclonal Antibody (HT301)
pEASY®-Blunt E2 Expression Kit (CE211)
研究背景
1902年，植物细胞全能性概念被提出，即植物细胞在适宜条件下可脱分化为全能干细胞，进而发育为完整植株。然而，其分子机制始终未解，2005 年《科学》杂志将“单个体细胞如何发育成完整植株”列为最具挑战的 125 个科学问题之一。
文章概述
研究团队首先建立了“诱导单细胞起源的体细胞胚胎发生”稳定体系，并系统寻找了全能干细胞的分子标记。利用单细胞测序、活体成像等技术，首次捕捉到单个植物细胞分裂全过程。研究发现，转录因子 LEC2 与 SPCH 协同作用激活生长素合成，使细胞内生长素特异性大量积累。这一过程促使原本注定发育为气孔的前体细胞脱离气孔发育路径，转化为全能干细胞，进而启动胚胎发生。研究进一步揭示，气孔前体细胞存在一个命运分岔点：细胞要么继续分化为气孔，要么进入“GMC-auxin”中间态。在这一中间态下，细胞通过染色质重塑、翻译调控和生长素信号的共同作用，激活胚胎发生程序，推动细胞命运从气孔分化转向全能干细胞，最终发育为完整植株。该研究在世界上首次全面解析了单个植物体细胞重编程形成全能干细胞并再生完整植株的分子机理，这一理论的解析不仅有助于理解植物细胞发育的根本规律，也为精准调控植物再生和定向改良作物性状提供了全新的思路与技术工具。
模式图展示气孔前体细胞的两条发育路径
全式金生物产品支撑
优质的试剂是科学研究的利器。全式金生物的反转录试剂（AE311）、qPCR 试剂（AQ101）、抗 HA 鼠单克隆抗体（HT301）、E2 平端克隆原核表达载体（CE211）助力本研究。产品自上市以来，凭借优异的性能，深受客户青睐，多次荣登知名期刊，助力科学研究。
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TransStart® Green qPCR SuperMix (AQ101)
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ProteinFind® Anti-HA Mouse Monoclonal Antibody (HT301)
本产品为抗 HA 标签鼠单克隆抗体，属 IgG2b 同型，免疫原为人工合成的 HA 标签多肽序列(YPYDVPDYA)。
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pEASY®-Blunt E2 Expression Kit (CE211)
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使用 EasyScript® One-Step gDNA Removal and cDNA Synthesis SuperMix (AE311) 产品发表的部分文章：
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• Tang S, Guo N, Tang Q, et al. Pyruvate transporter BnaBASS2 impacts seed oil accumulation in Brassica napus[J]. Plant Biotechnology Journal, 2022.(IF 13.26)
• Nie W, Wu G, Zhang J, et al. Responsive exosome nano‐bioconjugates for synergistic cancer therapy[J]. Angewandte Chemie International Edition, 2020.(IF 12.25)
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使用 TransStart® Green qPCR SuperMix (AQ101) 产品发表的部分文章：
• Tang L P, Zhai L M, Li J M, et al. Time-resolved reprogramming of single somatic cells into totipotent states during plant regeneration[J]. Cell, 2025. (IF 45.50)
• Li S, Tian Y, Wu K, et al. Modulating plant growth–metabolism coordination for sustainable agriculture[J]. Nature, 2018.(IF 41.58)
• Wu K, Wang S, Song W, et al. Enhanced sustainable green revolution yield via nitrogen-responsive chromatin modulation in rice[J]. Science, 2020.(IF 41.03)
• Sheng C, Zhao J, Di Z, et al. Spatially resolved in vivo imaging of inflammation-associated mRNA via enzymatic fluorescence amplification in a molecular beacon[J]. Nature Biomedical Engineering, 2022.(IF 26.80)
• Lei C, Kan H, Xian X, et al. FAM3A reshapes VSMC fate specification in abdominal aortic aneurysm by regulating KLF4 ubiquitination[J]. Nature Communications, 2023.(IF 16.60)
• Song N, Xu H, Liu J, et al. Design of a highly potent GLP-1R and GCGR dual-agonist for recovering hepatic fibrosis[J]. Acta Pharmaceutica Sinica B, 2022.(IF 14.90)
• Zhao J, Chu H, Zhao Y, et al. A NIR light gated DNA nanodevice for spatiotemporally controlled imaging of microRNA in cells and animals[J]. Journal of the American Chemical Society, 2019.(IF 14.69)
• Xue S, Zhang T, Wang X, et al. Cu, Zn dopants boost electron transfer of carbon dots for antioxidation[J]. Small, 2021.(IF 13.28)
• Tang S, Guo N, Tang Q, et al. Pyruvate transporter BnaBASS2 impacts seed oil accumulation in Brassica napus[J]. Plant Biotechnology Journal, 2022.(IF 13.26)
• Ren X, Li Y, Zhou Y, et al. Overcoming the compensatory elevation of NRF2 renders hepatocellular carcinoma cells more vulnerable to disulfiram/copper-induced ferroptosis[J]. Redox biology, 2021.(IF 11.79)
• Hu G, Long C, Hu L, et al. Blood chromium exposure, immune inflammation and genetic damage: Exploring associations and mediation effects in chromate exposed population[J]. Journal of Hazardous Materials, 2022.(IF 10.58)
• Zhang L, Xue S, Ren F, et al. An atherosclerotic plaque-targeted single-chain antibody for MR/NIR-II imaging of atherosclerosis and anti-atherosclerosis therapy[J]. Journal of Nanobiotechnology, 2021.(IF 10.44)
• Zhao K, Wang L, Qiu D, et al. PSW1, an LRR receptor kinase, regulates pod size in peanut[J]. Plant Biotechnology Journal, 2023.(IF 10.10)
使用 ProteinFind® Anti-HA Mouse Monoclonal Antibody (HT301) 产品发表的部分文章：
• Tang L P, Zhai L M, Li J M, et al. Time-resolved reprogramming of single somatic cells into totipotent states during plant regeneration[J]. Cell, 2025. (IF 45.50)
• Fan H, Quan S, Ye Q, et al. A molecular framework underlying low-nitrogen-induced early leaf senescence in Arabidopsis thaliana[J]. Molecular Plant, 2023.(IF 27.50)
• Yang L, Li D, Guo W, et al. WD40 protein-mediated crosstalk among three epigenetic marks regulates chromatin states and yield in rice[J]. Molecular Plant, 2025.(IF 24.10)
• Peng J, Zhang Q, Tang L P, et al. LEC2 induces somatic cell reprogramming through epigenetic activation of plant cell totipotency regulators[J]. Nature Communications, 2025.(IF 15.70)
• Li X, Wang X, Liu X, et al. A UFD1 variant encoding a microprotein modulates UFD1f and IPMK ubiquitination to play pivotal roles in anti-stress responses[J]. Nature Communications, 2025.(IF 15.70)
• Zheng C, Zhang B, Li Y, et al. Donafenib and GSK‐J4 Synergistically Induce Ferroptosis in Liver Cancer by Upregulating HMOX1 Expression[J]. Advanced Science, 2023.(IF 15.10)
• Li Q, Yang G, Ren B, et al. ZC3H14 facilitates backsplicing by binding to exon-intron boundary and 3′ UTR[J]. Molecular Cell, 2024.(IF 14.50)
• Zhang H, Huang C, Gao C, et al. Evolutionary‐Distinct Viral Proteins Subvert Rice Broad‐Spectrum Antiviral Immunity Mediated by the RAV15‐MYC2 Module[J]. Advanced Science, 2025.(IF 14.30)
• Ma A, Zhang D, Wang G, et al. Verticillium dahliae effector VDAL protects MYB6 from degradation by interacting with PUB25 and PUB26 E3 ligases to enhance Verticillium wilt resistance[J]. The Plant Cell, 2021.(IF 11.27)
• Wang B, Xue P, Zhang Y, et al. OsCPK12 phosphorylates OsCATA and OsCATC to regulate H2O2 homeostasis and improve oxidative stress tolerance in rice[J]. Plant Communications, 2023.(IF 10.50)
• Li W, Xiong Y, Lai L B, et al. The rice RNase P protein subunit Rpp30 confers broad‐spectrum resistance to fungal and bacterial pathogens[J]. Plant Biotechnology Journal, 2021.(IF 9.80)
• Wang Y, Shu H, Qu Y, et al. PKM2 functions as a histidine kinase to phosphorylate PGAM1 and increase glycolysis shunts in cancer[J]. The EMBO Journal, 2024.(IF 9.50)
• Yang Q, Tan S, Wang H L, et al. Spliceosomal protein U2B ″delays leaf senescence by enhancing splicing variant JAZ9β expression to attenuate jasmonate signaling in Arabidopsis[J]. New Phytologist, 2023.(IF 9.40)
• Fan G, Yang Y, Li T, et al. A Phytophthora capsici RXLR effector targets and inhibits a plant PPIase to suppress endoplasmic reticulum-mediated immunity[J]. Molecular Plant, 2018.(IF 9.33)
• Qi H, Yu J, Yuan X, et al. The somatic embryogenesis receptor kinase TaSERK1 participates in the immune response to Rhizoctonia cerealis infection by interacting and phosphorylating the receptor-like cytoplasmic kinase TaRLCK1B in wheat[J]. International Journal of Biological Macromolecules, 2023.(IF 8.20)
使用 pEASY®-Blunt E2 Expression Kit (CE211) 产品发表的部分文章：
• Tang L P, Zhai L M, Li J M, et al. Time-resolved reprogramming of single somatic cells into totipotent states during plant regeneration[J]. Cell, 2025. (IF 45.50)
• Yang L, Cheng Y, Yuan C, et al. The long noncoding RNA VIVIpary promotes seed dormancy release and pre-harvest sprouting through chromatin remodeling in rice[J]. Molecular plant, 2025. (IF 24.10)
• Xiao M, Wang B, Feng Y, et al. Three candidate 2-(2-phenylethyl) chromone-producing type III polyketide synthases from Aquilaria sinensis (Lour.) Gilg have multifunctions synthesizing benzalacetones, quinolones and pyrones[J]. Industrial Crops and Products, 2022. (IF 6.45)
• Zhao Y, Zheng Z, Zhang X, et al. Molecular Cloning and Expression Analysis of the Cryptochrome Gene CiPlant-CRY1 in Antarctic Ice Alga Chlamydomonas sp. ICE-L[J]. Plants, 2022. (IF 5.40)
